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Use of transwell system to analyze T lymphocyte migration across lymphatic endothelium

Preparation of antiserum and detection of proteins by Western blotting using the starlet sea anemone, Nematostella vectensis

Optimized immunofluorescence staining protocol to detect the nucleoporin Nup98 in different subcellular compartments

mRNA expression analysis by real-time PCR

Isolation of Treg cells and Treg cell suppression/death assay

Generation of mouse bone marrow-derived dendritic cells (BM-DCs)

Continuous long-term detection of live cell surface markers by ‘in culture’ antibody staining

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