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Continuous long-term detection of live cell surface markers by ‘in culture’ antibody staining

Generation of mouse bone marrow-derived dendritic cells (BM-DCs)

Isolation of Treg cells and Treg cell suppression/death assay

mRNA expression analysis by real-time PCR

Optimized immunofluorescence staining protocol to detect the nucleoporin Nup98 in different subcellular compartments

Preparation of antiserum and detection of proteins by Western blotting using the starlet sea anemone, Nematostella vectensis

Use of transwell system to analyze T lymphocyte migration across lymphatic endothelium

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