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Isolation of Treg cells and Treg cell suppression/death assay

Use of transwell system to analyze T lymphocyte migration across lymphatic endothelium

Optimized immunofluorescence staining protocol to detect the nucleoporin Nup98 in different subcellular compartments

Generation of mouse bone marrow-derived dendritic cells (BM-DCs)

mRNA expression analysis by real-time PCR

Continuous long-term detection of live cell surface markers by ‘in culture’ antibody staining

Preparation of antiserum and detection of proteins by Western blotting using the starlet sea anemone, Nematostella vectensis

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