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Generation of mutant mice with minimal off-target effects by microinjection of one-cell embryos with paired sgRNAs and Cas9 nickase
Identification of heart rate-associated loci and genes
Whole Mount X-gal Staining
Optically Highlighting Basement Membrane Components in C. elegans
Next generation engineering of conditional mouse alleles with loxP and FRT sites by dual RMCE.
Validation of precise sequences of computationally identified microRNAs by miR-RACE
Isolation of deletion alleles of C. elegans by G4-DNA induced mutagenesis
Identification of genes directly regulated by a transcription factor in rice
Deathless transgenesis: a new spermatogonia mediated in vivo approach for generation of transgenic mice
Monitoring protein synthesis by fluorescence recovery after photobleaching (FRAP) in vivo
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