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Optimized immunofluorescence staining protocol to detect the nucleoporin Nup98 in different subcellular compartments

Preparation of antiserum and detection of proteins by Western blotting using the starlet sea anemone, Nematostella vectensis

Preparation of peptidoglycan-depleted soluble microbial extract

Production of polyclonal antibodies against protein antigens purified by electroelution from SDS-polyacrylamide gel

Production of recombinant hBD-1 in Escherichia coli and its specific polyclonal antibody in rabbits

Quantifying in vivo recruitment of naïve CTL towards individual dendritic cells by standard immunofluorescence histology

Quantitative Procedure to Analyze Nuclear β-Catenin Using Immunofluorescence Tissue Staining

Rapid enzyme-linked immunosorbent assay technique by ultrasound waves in a sonicator bath

Retroviral transduction in cultured murine basophils

RNAi in human monocyte-derived dendritic cells using shRNA vectors

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