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mRNA expression analysis by real-time PCR

Mouse Mutagenesis Using N-Ethyl-N-Nitrosourea (ENU)

Morpholino-mediated gene knockdown in the early mouse embryo

Microinjection of dsRNA into Mouse Oocytes and Early Embryos

Measuring fatty acid oxidation in tissue homogenates

Isolation of Treg cells and Treg cell suppression/death assay

Isolation of T cells and dendritic cells from peripheral intestinal tissue, Peyer’s Patches and mesenteric lymph nodes in mice after intestinal manipulation

Isolation of neural crest cells derived from human embryonic stem cells

Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)

In situ protocol for embryos and juveniles of Convolutriloba longifissura

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