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A one-step kinetics-based rapid sandwich ELISA procedure using leach-proof antibody immobilization for the highly-sensitive detection of human fetuin A

Co-immunoprecipitation of Platelet Factor 4 and RANTES from human platelets

A protocol to detect apoptotic dendritic cells in murine lymph nodes using multiphoton microscopy

Generation of mouse bone marrow-derived dendritic cells (BM-DCs)

mRNA expression analysis by real-time PCR

Preparation of peptidoglycan-depleted soluble microbial extract

In vitro analysis of cytotoxic T cell recruitment mediated by the DC-derived chemokine CCL17

Quantifying in vivo recruitment of naïve CTL towards individual dendritic cells by standard immunofluorescence histology

Co-immunoprecipitation of human mitochondrial proteases AFG3L2 and paraplegin heterologously expressed in yeast cells

Production of polyclonal antibodies against protein antigens purified by electroelution from SDS-polyacrylamide gel

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