New protocol
Z - A Newest

Production of recombinant hBD-1 in Escherichia coli and its specific polyclonal antibody in rabbits

Production of polyclonal antibodies against protein antigens purified by electroelution from SDS-polyacrylamide gel

Preparation of peptidoglycan-depleted soluble microbial extract

Preparation of antiserum and detection of proteins by Western blotting using the starlet sea anemone, Nematostella vectensis

Optimized immunofluorescence staining protocol to detect the nucleoporin Nup98 in different subcellular compartments

mRNA expression analysis by real-time PCR

Mouse meninges isolation for FACS

Monitoring endothelial cell development and migration in the embryonic CNS

Modified Immunohistochemical Protocol for Paraffin Embedded Sections

Measurement of gastrointestinal and colonic transit in mice

Scientific Protocols is part of the Reproducibility Initiative | Contact | API