Authors: Stephan Christel & Mark Dopson
Trace element solution for Mineral Salts Medium (MSM, see separate protocol http://www.nature.com/protocolexchange/protocols/3809). To be added when medium is not supplied with mineral. Add 1 ml of 100x Stock TE solution into 99 ml of MSM.
For 100x Stock TE solution:
10 mg/l Na2SeO4
Add the constituents into a final volume of 1 liter of water (pH pre-adjusted to around 2.5), one at a time, dissolving before adding the next.
Adjust the pH to 1.5 with sulfuric acid (and/or sodium hydroxide)
about 30 minutes
TE solution should be clear and almost colorless. Strong coloration indicates too high pH.
When encountering difficulties during dissolution of a reagent, try adding some more sulfuric acid.
Stephan Christel & Mark Dopson, Systems Biology of Extreme Microorganisms, Dopson, Linnaeus University
Correspondence to: Stephan Christel ([email protected])
Source: Protocol Exchange (2015) doi:10.1038/protex.2015.032. Originally published online 24 March 2015.