Authors: Mark Dopson & Stephan Christel
Medium for growth of extreme acidophilic microorganisms. This medium does not contain trace elements. When not working on mineral, addition of trace element (TE) solution is necessary, see separate protocol. http://www.nature.com/protocolexchange/protocols/3811
For 1x Medium:
For 10x Stock:
Add the constituents into a final volume of 1 liter of water, one at a time, dissolving before adding the next.
Adjust the pH to 2.0 with sulfuric acid (and/or sodium hydroxide)
15 minutes + autoclaving time
Mark Dopson & Stephan Christel, Systems Biology of Extreme Microorganisms, Dopson, Linnaeus University
Correspondence to: Stephan Christel ([email protected])
Source: Protocol Exchange (2015) doi:10.1038/protex.2015.031. Originally published online 24 March 2015.