scientificprotocols authored about 7 years ago
Authors: Naxin Jiang, Nguan Soon Tan, Bow Ho & Jeak Ling Ding
The lysis of the red blood cells can be monitored by the release of hemoglobin to the extracellular environment. After removing the intact RBC by centrifugation, the absorbance at 404 nm of the supernatant reflects the hemolysis. The RBC lysate obtained by total disruption by 1% Triton- X100, is used as a positive control.
The RBC lysis by bacterial hemolysin is measured as described1 with modification.
Hemolysin producing microbes, such as S. aureus, lyses the RBC and the typical A404= 1.2 – 1.6.
Figure 1: Hemolysis of RBC by S. aureus laboratory strains PC1839 and AK3.
S. aureus strains, PC 1839 and AK3, at cell density of 107 cfu/ml, rupture the RBC within 5 min.
Respiratory protein–generated reactive oxygen species as an antimicrobial strategy, Naxin Jiang, Nguan Soon Tan, Bow Ho, and Jeak Ling Ding, Nature Immunology 8 (10) 1114 - 1122 26/08/2007 doi:10.1038/ni1501
Naxin Jiang, Nguan Soon Tan, Bow Ho & Jeak Ling Ding, National University of Singapore
Source: Protocol Exchange (2007) doi:10.1038/nprot.2007.483. Originally published online 31 October 2007.