Analytical Chemistry Plants Isolation Purification and Separation

scientificprotocols authored about 8 years ago

Authors: Yalin Tang, Qian Shang, Junfeng Xiang, Qianfan Yang, Qiuju Zhou, Lin Li, Hong Zhang, Qian Li, Hongxia Sun, Aijiao Guan, Wei Jiang & Wei Gai


This protocol presents the screening of ligand(s) from medicinal plant extracts based on target recognition by using NMR spectroscopy. A detailed description of sample preparation and analysis process is provided. NMR spectroscopies described here are 1H NMR, diffusion-ordered spectroscopy (DOSY), relaxation-edited NMR, 1H–13C HSQC and HMBC. This method includes three steps: First, investigate the NMR spectroscopy properties of target and choose the suitable spectral editing NMR method; second, judge the existence of ligand(s) and determine the proton signals of ligand(s) in the extracts; third, verify the structure(s) of the ligand(s). This method allows the direct structural identification of ligand(s) from medicinal plant extracts without separation and purification. Thus it provides a very promising strategy for the fast screening of lead components based on target recognition.


Plant-derived agents, owing to their diversity in structure and bioactivity1-6, play critical roles in pharmaceutical research. They provide lead compounds for biopharmaceutical technology and supply an economical ways for the discovery of new drugs. The drugs, which are used widely in modern medicine, mostly derived from natural compounds and their derivatives. For example, aspirin was originated from salicylic acid in the bark of the willow tree (Salix species), which is used traditionally to treat fever and inflammation in many cultures worldwide7. The successes of the early “blockbuster” drugs set the stage for ongoing drug discovery efforts from medicinal plants.

The screening of bioactive ingredients based on targets recognition attracts researchers’ attention8-10. Nevertheless, these methods can hardly be applied in direct screening of bioactive ingredients from plants extracts which compose of various compounds with different contents. Currently screening from plant extracts against specific targets always follows the strategy of “Isolation  Structure identificationActivity confirmation”. Yagura et al.11 isolated isosalipurposide for anticarcinogenic compounds in the Uzbek medicinal plant by bioactivity-directed fractionation. Bai et al.12 screen the G-quadruplex ligands from Kalopanax septemlobus (Thunb) Koidz extract by high performance liquid chromatography (HPLC). Compared with these methods, our method will accelerate the screening process of lead components greatly by providing the structural information of bioactive ingredients without isolating the ligand(s) molecules experimentally. Thus it provides chemists and pharmacologists with useful info to optimize lead compound for the final drug in a more rapid way.

The implementation of our goal benefits from the powerful NMR techniques. NMR has now become an important tool in drug discovery13-19, owing to its advantage in structure identification. But the complexity and overlapping of signals in complex systems bring difficulties in the recognition of spectra. With the development of spectral editing NMR techniques, selected useful information also can be obtained. Among these techniques, diffusion-ordered spectroscopy (DOSY) and relaxation-edited methods are two successful examples20-23, which could provide means for signal filtering and selecting, and consequently achieve “virtual separation”. In this protocol, DOSY and relaxation-edited NMR techniques are used to screen the bioactive ingredients interacting with target in medicinal plant extracts.

Our method has drawn a profound attention on screening bioactive ingredients from medicinal plants and there are many reviews commenting on it24, 25. The reviewer of Angewandte Chemie International Edition pointed out that the predominance of this method was integration screening and structure identification, which carried out the fast screening and structure identification of bioactive ingredients in natural plant extracts. Nature China highlighted this method and commented on it with Screening Methods: Looking for Ligands as title.

In order to introduce this method to more researchers who devote to search the bioactive ingredients in natural plant extracts and provide convenience for their works, it is necessary to describe this method in detail and define standard protocol in order to ensure the correct usage of this method. Here we take the screening and identifying ligands from the extract of Phellodendron chinense Schneid cortexes (PE)26 based on G-quadruplex and the extract of Flos Lonicera Japonica (FLJ) based on Avian Influenza Polymerase Protein PAC by using NMR as examples to define well-tested procedures.

Experimental design

The biomolecules which are reported to have important physiological significance can be used as targets. To be specific, our protocol takes G-quadruplex and Avian Influenza Polymerase Protein PAC (carboxyterminal domain of PA) as examples.

G-quadruplexes27,28 can inhibit the activity of telomerase29 and play an important role in suppression of carcinogenesis30-33. The ligands of G-quadruplex have the potential for the arrest of cancer-cell growth and may be potentially valuable as antitumor drugs34-36. The interaction of G-quadruplex and its ligand affects the chemical shifts of imino protons in G-quadruplex, which locate low-field region (10-12 ppm) of NMR spectra.

Additionally, the ligand of protein also can be obtained by using spectrally-filtered editing NMR techniques. For PAC, the drugs discovered by it may be effective against most influenza strains less susceptible to drug resistance due to the high conservation of the active sites in PAC37.

The medicinal plant extracts which are reported to treat some kind of disease can be used as candidates for the corresponding targets. PE is chosen as a candidate because it is reported to be benefit for the treatment of cancer and contain G-quadruplex ligands38. FLJ is chosen as a candidate because it is reported to be benefit for the treatment of viral infection such as influenza A virus39. The concentration of medicinal plant extracts depends on the concentration of bioactive ingredients. The concentration of medicinal plant extracts should be benefit for observing the obvious changes from the characteristic signals of the targets.

There are three steps in this process (Figure 1): First, it is necessary to choose the suitable NMR sequence according to the target. To be specific, if there are not clear and characteristic signals of target in NMR spectroscopy, relaxation-edited NMR is preferred. Otherwise, DOSY is suitable. For example, G-quadruplex has the characteristic peaks around δ 10-12 ppm which are clear and not easy to be interfered by other components. In this case, DOSY can be chose. But for proteins they usually have high molecular weights, thus their peak profiles are broadened and there are uneasy to be identified. In this case, relaxation-edited NMR can be chose. Second, judge the existence of ligand(s) and determine the proton signals of ligand(s) by spectral editing NMR techniques in the extracts. For example, G-quadruplex is taken as target in DOSY. As long as the ligand(s) binds to G-quadruplex, the chemical shifts and intensities of imino protons of G-quadruplex which are clear and characteristic signals of G-quadruplex change and the change is easy to be detected. Then extract the signal of ligand(s) by DOSY. In the relaxation-edited NMR spectra, through the comparison with the spectrum of extract without PAC, the peak of ligand can be picked out. Third, further structural illumination is used to identify the ligand(s) by 1H-13C HSQC and HMBC from the signal obtained in step 2.

Limits of applicability and practical considerations

If there were bioactive ingredients in medicinal plant extracts and their structures were unknown, this protocol could be employed to find them out. As long as the signals of the ingredients which could interact with the target could be distinguished in NMR spectroscopy, this protocol could be used to identify their structures without isolation.

Although it provided a fast approach to structurally determine bioactive ingredients, this method had some limitations.

Firstly, the content of bioactive ingredients in the extracts is a key factor that influences the result. For example, the G-quadruplex ligand detected in PE is about 0.06% (mass concentration). If there is more than one ligand in test extracts, the content of each ligand dominates the priority of ligands identified in test extracts in this protocol. For a multiligand system such as extracts of Coptis chinensis Franch rhizomes (CE)26 which contains at least two ligands (berberine and palmatine) with similar G-quadruplex binding ability, the ligand with the higher content tend to be identified and that with lower content may be omitted.

The second factor is the requirement of the target. Because our protocol bases on NMR techniques, generally the target must meet the demands in NMR experiments, such as the concentration and molecular weight40. To be specific, it requires the relatively high concentration (mM level) of target when the molecular weight of target is about ten thousand owing to the sensitivity of DOSY.



  1. DNA d(TTGGGTT) (Tsingke Biotechnology Co., Ltd)
  2. TSP (3-(trimethylsilyl) propionic acid-d4 sodium salt), berberine (Sigma Co.)
  3. Lentinan extracts (Shanghai kangzhou Fungi extract Co., Ltd)


  1. Avance 600 NMR spectrometer (Bruker-Biospin). 600 MHz is not the only frequency that can be used in this method, but it is a good compromise for sensitivity and dispersion versus capital cost.
  2. A 5 mm BBI probe capable of delivering z-field gradients.
  3. Eppendorf pipette and pipette tips or similar


G-quadruplex In practice, the sample preparation for this experiment is simple. The preparation of G-quadruplex d(TTGGGTT)4 is the same as the procedure described in the literature41. G-quadruplex d(TTGGGTT)4 was formed by dissolving primer d(TTGGGTT) in phosphate buffer (10 mM K2HPO4/KH2PO4, 90% H2O/10% D2O, pH 7.0). The solution was equilibrated at room temperature for 24 h before experiments. The absorbance of DNA d(TTGGGTT) at 260nm is obtained by absorption spectroscopy. It is hypothesized that all DNA d(TTGGGTT) can form d(TTGGGTT)4 and the concentration of d(TTGGGTT)4 is . The minimum concentration of G-quadruplex d(TTGGGTT)4 is 0.25 mM. Under the concentration the characteristic signals of d(TTGGGTT)4 are easily distinguished in NMR spectroscopy.

PAC Methods for the preparation of PAC protein were previously described5. Briefly, residues 257–716 of the PA subunit of avian H5N1 influenza A virus (A/goose/Guangdong/1/96) were cloned into a pGEX-6p vector (GE Healthcare) and transformed into Escherichia coli strain BL21. Cells were cultured in LB medium at 37℃ with 100 mg/L of Ampicillin. When the OD600 reached 0.6–0.8, the culture was induced with 0.5 mM isopropyl-thio-Dglactosidase (IPTG) at 16℃. After 20 hours of incubation, the cells expressing PAC were harvested and combined by centrifugation at 5000 rpm for 10 min. Recombinant protein was purified with a glutathione affinity column (GE Healthcare). Glutathione S-transferase (GST) was cleaved with PreScission protease (GE Healthcare), and the protein complex was further purified by Q sepharose FF ion exchange chromatography and Superdex-200 gel filtration chromatography (GE Healthcare).

Extracts of Phellodendron chinense Schneid cortexes (PE) A 500 mL three-neck flask is equipped with a magnetic stirrer, thermometer, heating mantle, and a reflux condenser, 60 g powder of Phellodendron chinense Schneid cortexes and 300 ml ethanol-water solution (the volume ratio of water and ethanol was 1:3) are added to the flask. Then the mixture is heated up to 80 degrees Celsius with stirring for 5 h. The crude product was collected by filtration, and dried in a vacuum desiccator at 60 degrees Celsius for 48 h. 31.5 g product is obtained.

Extracts of Flos Lonicera Japonica (FLJ) 10 g powder of FLJ was reflux extracted with 300 mL 15% ethanol-water solution for 5h. The residue was then removed by filtration, and the filtrate was desiccated in a vacuum desiccator at 60 ºC for 48 h. 1 g of FLJ extracts was finally obtained, respectively.



NMR setup The high-resolution NMR spectroscopy could be used for screening and identifying new bioactive ingredients42. In the following, experimental methods are described using a Bruker NMR spectrometer as an example. Some common pulse techniques can be employed to screen bioactive ingredients with futher structure identification, such as standard Bruker pulse program p3919gp, stebpgp1s19, cpmgpr1d, hsqcetgp and hmbcgplpndqf.

Setup on NMR spectrometers of other vendors will require adjustment accordingly. In order to obtain maximum benefit from acquiring NMR spectra, the parameters need to be set accurately. Generally, all NMR experiments should be acquired at 298.2K.

Homogenization of the magnetic field (‘shimming’) for samples is important for conventional NMR probes. This can be done with TopShim on Bruker spectrometers (TopSpin 2.1). The homogenization of the magnetic field can be confirmed by the peak profile of TSP which is a single peak at 0ppm. The number of scans per experiment for different medicinal plant extracts can be adjusted according to the signal-to-noise ratio of the components of interest, but this will have an impact on the overall experiment time per sample.

Water suppression When observing protons, the dynamic range of the detection can be strongly limited by the size of the water peak and the results in the signal loss for low concentration substances. Hence, the water peak has to be suppressed by using the pulse program p3919gp that applies 3–9–19 pulses with gradients for water suppression43 to improve the signal-to-noise ratio for the detection of ligands. Other spectrometer manufacturers provide the same pulse sequences, but they have different names and syntax; please discuss with the supplier.

The parameters are optimized to give optimum suppression of the water resonance without reducing the signal intensity of the compound signals next to it. It is recommended to automatically adjust the receiver gain before acquiring the spectra.

All NMR experiments require correct setting of 90° pulse length. It is a key to determine the offset of the water signal for the water suppression.

DOSY To get the actual gradient strength in absolute values, it is necessary to obtain a gradient calibration constant, which is used by the AU program dosy to calculate and store the list (difflist) containing absolute gradient strength values. This list is used by the processing tools to calculate the correct diffusion constants.

The gradient strength (g), diffusion time big delta (Δ) and diffusion gradient length little delta (δ) are important factors in DOSY experiment. It is necessary to optimize these three parameters to detect the whole decay function properly. Selecting the right values for Δ or δ is important to get good diffusion constants with less error.

Using 1D versions of the diffusion pulse program for optimizing the Δ (d20) and δ (p30), experiments are carried out by comparing two spectra with different amplitude (gpz6) 2% and 95%. The parameters are set when the signal decay goes down to 5% residual signal. The comparison is convenient in dual display.

Cpmgpr: The relaxation-edited NMR experiments utilized a [D/pre-saturation-90x-(Δ-180y-Δ)n-acquire] pulse sequence, in which the CPMG sequence was used for the spin-lock.

Structure identification using 2D NMR spectroscopy Two-dimensional heteronuclear NMR spectroscopy is employed for identifying the connectivity between signals. 1H–13C HSQC and HMBC NMR spectra are used for structural identification44, as they highlight connectivities of protons directly attached to the carbon atoms in a molecule.


  • Sample preparation ● TIMING 10–12 min
  1. Dissolving target in appropriate buffer solution and adjust the concentration. For G-quadruplex d(TTGGGTT)4, the concentration is 0.25 mM. For PAC, the concentration is 8.7×10-3mM.
    • ▲ CRITICAL STEP In the work with G-quadruplex, the formation of G-quadruplex is of paramount importance. Usually triethylamine used in the synthesis of d(TTGGGTT) usually affects the binding between G-quadruplex and ligands. Thus triethylamine needs to be removed by dialysis before the usage of d(TTGGGTT) in this protocol.
  2. For DOSY, make the mixture of d(TTGGGTT)4 and PE and ensure the concentration is 0.25 mM and 3.5 mg mL-1, respectively. For relaxation-edited NMR, prepare two samples. One is the mixture of PAC and FLJ and the concentration is 8.7×10e-3mM and 3.5 mg mL-1, respectively. The other is FLJ and the concentration is 3.5 mg mL-1.
  3. Put the above samples into 5mm NMR tubes and the total volume is 5×10-4L for each sample, respectively.
    • NMR setup ● TIMING 15–17 min
  4. Insert the NMR tube into the spinner and measure the correct height with the gauge.
  5. Enter the and wait for 5–10 s before entering the button. The sample is now positioned in the probe.
  6. Wait for 5–10 minutes for the temperature equilibrium.
  7. Optimization of NMR spectrometer: (1) tune and match the probe; (2) lock onto the lock solvent; and (3) shim the sample using the lock level.
  8. Determine the sample-specific settings for NMR pulse sequences: (1) Set the offset value to the HDO resonance. (2) Determine the 90° pulse length at a given power level. (3) Re-adjust the frequency offset for water signal suppression, if necessary.
    • ▲ CRITICAL STEP The suppression of water properly is beneficial for the observation of tiny constituent.
    • NMR acquisition ● TIMING 24 h
  9. First, 1D p3919gp sequence (option A) is used to test whether the extracts contain G-quadruplex ligand(s). (TIMING 12-15 min) Second, DOSY (option B) is used to pick out the peak of ligand. (TIMING 2 h) Third, based on the peak of ligand, HSQC and HMBC (option C) are used to identify the structure of ligand. (TIMING 24 h) Adjust the receiver gain per sequence and sample by using automatic receiver gain adjustment. Processing parameters: if not mentioned otherwise, the one-dimensional spectra are generally processed by applying a line broadening of 0.3–1 Hz.
    • (A) 1D p3919gp sequence (Bruker spectrometer: p3919gp)
      • (i) The parameters are set as follows: spectral width = 20 ppm; number of time domain data points = 32 k; relaxation delay (RD) = 2.0 s; number of scans = 128; and the receiver gain is set to fill the digitizer as closely as possible. This results in a total acquisition time of about 11 min per sample.
      • (ii) For processing, a target spectral resolution is difficult to define, but typically with a line-broadening of 1 Hz. (TIMING 1 min)
    • (B) DOSY (Bruker terminology: stebpgp1s19)45
      • (i) Acquire diffusion-edited spectra using a pulse sequence with stebpgp1s19. The settings are relaxation delay = 2 s; number of scans = 128 (or higher, depending on requirements); data points in the F2 dimension = 32 k; data points (gradient strengths) in the F1 dimension = 32; spectral width = 20 ppm; diffusion delay Δ = 0.1 s; the gradient length δ = 5.6 ms; line broadening factor in F2 dimension = 0.3 Hz. The data analyses were applied to the raw experimental data using the standard 2D DOSY processing protocol in TOPSPIN (Bruker, Version 2.1) software with logarithmic scaling in the F1 (diffusion coefficient) dimension.
    • (C) Heteronuclear correlation spectroscopy (Bruker terminology: hsqcetgp and hmbcgplpndqf)46
      • (i) Acquire 1H–13C HSQC spectrum using sensitivity improvement with echo-anti echo-TPPI. GARP decoupling of 13C is carried out during the acquisition time. 0.1 μs trim pulses are employed in the INEPT transfer and gradients in back-INEPT. The parameters are as follows: resolution in F2/F1 = 2 k/512 (depending on available experiment time); number of scans = 128 (depending on experiment time); number of dummy scans = 16; sweep width in F2/F1 = 20 ppm/170 ppm in hsqcetgp, and relaxation delay = 2 s. C-H coupling constant is 145 Hz. 1024×1024 points are used in Fourier transformation.
      • (ii) Collect 1H-13C phase-sensitive (TPPI) HMBC spectra. GARP decoupling of 13C is carried out during the acquisition time. The parameters are as follows: resolution in F2/F1 = 2 k/512 (depending on available experiment time); number of scans = 128 (depending on experiment time); number of dummy scans = 16; sweep width in F2/F1 = 20 ppm/250 ppm in hmbcgplpndqf, and relaxation delay = 2 s. The long range C-H coupling constant for HMBC is 6.25 Hz. 1024×1024 points are used in Fourier transformation.

Optional: 9| First, 1D cpmgpr1d sequence (option A) is used in FLJ in the absence (a) and presence (b) of PAC with different spin-lock time.(generally 100-1500ms) (TIMING 24-30 min) Second, the difference spectrum is made with (a) and (b) and find out the changed peaks which belong to ligand. (5min) Third, based on the peaks of ligand, HSQC and HMBC (option C) are used to identify the structure of ligand. (TIMING 24 h) Adjust the receiver gain per sequence and sample by using automatic receiver gain adjustment. Processing parameters: if not mentioned otherwise, the one-dimensional spectra are generally processed by applying a line broadening of 0.3–1 Hz. (A) 1D cpmgpr1d sequence (Bruker spectrometer: cpmgpr1d) (i) The parameters are set as follows: pre-saturation water suppression was applied in pre-acquisition delay (D = 3 s); Δ = 1.5 ms, and 2×n×Δ = total spin-lock time; spectral width = 20 ppm; number of time domain data points = 64 k; relaxation delay (RD) = 2.0 s; number of scans = 32; and the receiver gain is set to fill the digitizer as closely as possible. This results in a total acquisition time of about 1-30 min per sample depending on the spin-lock time.


  • Steps 1–3 Sample preparation: 10–12 min
  • Steps 4-8 NMR setup: 15–17 min
  • Step 9 NMR acquisition: 24 h


  • Step 8
    • The main problems can be avoided by ensuring that the water offset and 90° pulse length are adjusted on samples (90° pulse length needs to be adjusted on a sample-by-sample basis).
  • Step 9
    • The receiver gain requires automatic receiver gain adjustment. This will avoid problems with baseline rolling artifacts.

Anticipated Results

Accurate application of the steps in this protocol will lead to consistent NMR spectra. Once these spectra have been obtained, they will require phasing and baseline correction before being subjected to further analysis.

The 1H spectra of G-quadruplex d(TTGGGTT)4 with different plant extracts [with or without G-quadruplex ligand(s)], are shown in Figure 2.

The evident differences in the imino region (10–12 ppm) in the 1H NMR spectra of free and bound G-quadruplexes47 can be utilized as a spectroscopic means indicating the existence/nonexistence of G-quadruplex ligand(s) in the test extracts. As shown in Figure 2, when lentinan (without G-quadruplex ligand) is added into the d(TTGGGTT)4, the chemical shift and intensity of G3-G5 are unchanged. However, when PE (with G-quadruplex ligand) is added into the d(TTGGGTT)4, a clear upfield shift of the guanine imino proton resonance signals was observed and signal intensity, especially that of G5 dropped greatly.

Figure 3 shows that DOSY analysis of a mixture of PE and d-(TTGGGTT)4. Based on the principle of DOSY, if the component binds to d-(TTGGGTT)4, the component has the same diffusion coefficient with d-(TTGGGTT)4. The characteristic peaks of d-(TTGGGTT)4 are around δ 10-12. In Figure 3, the peak around δ 9 ppm has the same ordinate with the peaks around δ 10-12 which means the components from δ 9 ppm have the same diffusion coefficient with d-(TTGGGTT)4. Additionally no such peak appears in the spectrum of the G-quadruplex alone. This result, together with a consideration of the sharp drop in the diffusion coefficient of the proton that resonates around δ 9 ppm in the two samples (4.8×10-10 m2 s-1 in PE and 1.4×10-10 m2 s-1 in a mixture of PE and d-(TTGGGTT)4 respectively), led us to reasonably conclude that this peak resulted from the compound in PE bound to G-quadruplex.

According to the peak at δ 9 ppm, the structure of the berberine could be recognized by analyzing the HSQC and HMBC spectra of PE26. (Supplement)

The relaxation-edited NMR spectra of FLJ in the absence and presence of PAC and the difference spectrum are shown in Figure 4.

In order to identify the PAC ligand from the mixtures, relaxation-edited NMR with Carr-Purcell-Meiboom-Gill (CPMG) spin-lock was applied to FLJ extract in the absence and presence of PAC. As shown in Figure 4b, the application of CPMG spin-lock will reduce the ligand peaks due to its relatively fast R2 upon binding. However, these signals were not influenced in the absence of target (Figure 4a). This difference was highlighted in the difference spectrum (Figure 4c). These attenuated peaks can be ascribed to the PAC ligand in the extract. Thus, the ligand peaks can be “picked out” from the mixture without previous isolation. The next step is to identify which molecule in the mixture is bound to PAC.

Based on the characteristic signals picked out by relaxation-edited NMR, structure elucidation was carried out using the subsequent 2DNMR experiments, such as HSQC, HMBC, and total correlation spectroscopy (TOCSY). Followed by proton-carbon connections, proton-carbon long-range correlations, and proton-proton correlations provided by these 2DNMR experiments, the molecular frame of the ligand can be built. The PAC ligand can be then identified to be chlorogenic acid.


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This work is supported by the Major Research Program of the National Natural Science Foundation of China (Grant No. 91027033), the General Program of the National Natural Science Foundation of China (Grant No. 81072576) and the Key Program of the Chinese Academy of Sciences (Grant No. KJCX2-EW-N06-01).


Figure 1: The steps in this protocol

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Figure 2: The 1H NMR spectra of a) d(TTGGGTT)4, b) a mixture of d-(TTGGGTT)4 and lentinan extract (without G-quadruplex ligand), and c) a mixture of d(TTGGGTT)4 and PE (with G-quadruplex ligand, berberine).

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The concentrations of d(TTGGGTT)4, lentinan extract, and PE are 0.25 mM, 3.50 mg mL-1, and 1.0 mg mL-1, respectively. The region of 10.5–12.0 ppm is broadened and the imino proton resonance signals are labeled as G3–G5. (Reproduced with permission from ref. 25)

Figure 3: DOSY analysis of a mixture of PE (1.0 mg mL-1) and d- (TTGGGTT)4 (0.25 mM). The peak of the d(TTGGGTT)4 ligand(s) is designated by “↓” in the F2 projection. (Reproduced with permission from ref. 25)

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Figure 4: The relaxation-edited NMR spectra of FLJ in the absence (a) and presence (b) of PAC and the difference spectrum (c) of a and b.

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The spin-lock time in a and b were both 1500 ms. Contents of FLJ extract were 3.3mg mL-1, and concentration of PAC was 8.7 × 10-3mM. The water peak located at δ 4.8, and 1mM of TSP was added to the sample as a reference (δ 0). The region of δ 6.2-7.8 is broadened, and the ligand peaks attenuated upon the addition of the target were marked with “” in plot c*.

Associated Publications

  1. Screening Potential Antitumor Agents from Natural Plant Extracts by G‐Quadruplex Recognition and NMR Methods. Qiuju Zhou, Lin Li, Junfeng Xiang, Yalin Tang, Hong Zhang, Shu Yang, Qian Li, Qianfan Yang, and Guangzhi Xu. Angewandte Chemie International Edition 47 (30) 5590 - 5592 14/07/2008 doi:10.1002/anie.200800913
  2. Screening Anti-influenza Agents that Target Avian Influenza Polymerase Protein PAC from Plant Extracts Based on NMR Methods. Lin Li, Sheng-Hai Chang, Jun-Feng Xiang, Qian Li, Huan-Huan Liang, Jian Li, Hong-Juan Bao, Ya-Lin Tang, and Ying-Fang Liu.* Chemistry Letters* 40 (8) 801 - 803 doi:10.1246/cl.2011.801
  3. Screening α-glucosidase inhibitors from mulberry extracts via DOSY and relaxation-edited NNR. Qian Shang, Jun-Feng Xiang, and Ya-Lin Tang. Talanta 97 () 362 - 367 doi:10.1016/j.talanta.2012.04.046
  4. Fast screening and structural elucidation of G-quadruplex ligands from a mixture via G-quadruplex recognition and NMR methods. Qiuju Zhou, Lin Li, Junfeng Xiang, Hongxia Sun, and Yalin Tang. Biochimie 91 (2) 304 - 308 doi:10.1016/j.biochi.2008.10.011
  5. Fishing potential antitumor agents from natural plant extracts pool by dialysis and G-quadruplex recognition. Qian Shang, Jun-Feng Xiang, Xiu-Feng Zhang, Hong-Xia Sun, Lin Li, and Ya-Lin Tang. Talanta 85 (1) 820 - 823 doi:10.1016/j.talanta.2011.04.011
  6. Direct screening of G-quadruplex ligands from Kalopanax septemlobus (Thunb.) Koidz extract by high performance liquid chromatography. Ge Bai, Xueli Cao, Hong Zhang, Junfeng Xiang, Hong Ren, Li Tan, and Yalin Tang. Journal of Chromatography A 1218 (37) 6433 - 6438 doi:10.1016/j.chroma.2011.07.028
  7. Screen Anti-influenza Lead Compounds That Target the PAC Subunit of H5N1 Viral RNA Polymerase. Lin Li, Shenghai Chang, Junfeng Xiang, Qian Li, Huanhuan Liang, Yalin Tang, Yingfang Liu, and Jun Liu. PLoS ONE 7 (8) 24/08/2012 doi:10.1371/journal.pone.0035234
  8. NMR identification of anti-influenza lead compound targeting at PAC subunit of H5N1 polymerase. Lin Li, Sheng Hai Chang, Jun Feng Xiang, Qian Li, Huan Huan Liang, Ya Lin Tang, and Ying Fang Liu. Chinese Chemical Letters 23 (1) 89 - 92 doi:10.1016/j.cclet.2011.09.006

Author information

Yalin Tang, Tang yalin

Qian Shang, Junfeng Xiang, Qianfan Yang, Qiuju Zhou, Lin Li, Hong Zhang, Qian Li, Hongxia Sun, Aijiao Guan, Wei Jiang & Wei Gai, Unaffiliated

Correspondence to: Yalin Tang ([email protected])

Source: Protocol Exchange (2012) doi:10.1038/protex.2012.060. Originally published online 7 December 2012.

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