Authors: Constance Jeffery
Simple directions to prepare frozen permanents of E. coli or other bacterial cultures that can be stored for years at -80°C.
This is a simple protocol for preparing frozen permanents of E. coli or other bacterial cells. The frozen permanents can be stored for years at -80°C.
Overnight to grow culture plus about 15 minutes to complete the rest of the protocol.
Frozen permanents should be good for years if stored at -80°C.
Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein. Forum H. Bhatt and Constance J. Jeffery. Journal of Visualized Experiments 03/12/2010 doi:10.3791/2134
Constance Jeffery, Connie Jeffery's Lab
Correspondence to: Constance Jeffery ([email protected])
Source: Protocol Exchange (2011) doi:10.1038/protex.2011.228. Originally published online 14 June 2011.