Authors: Sudha Manickam
Pulses are major constituents of human diet and are mainly grown in marginal lands under rainfed conditions. Among the pulses, blackgram and mungbean are the major pulses contributing much for the total production. Mungbean (Vigna radiata (L.) Wilczek) (2n=22), primarily an inbreeding species is cultivated throughout tropical Asia including India which accounts for 45% of the world production. Vigna species are an important source of protein for people, particularly in tropical Africa and Asia and several Vigna species have been domesticated in Asia. Species in Leguminosae (Fabaceae) genus Vigna subgenus Ceratotropis are called the Asian Vigna because of their natural distribution. Recently the taxonomy of the subgenus has been revised (Tomooka et al., 2002c). On the basis of these taxonomic revisions, a new taxonomic system was proposed for subgenus Ceratotropis that consists of 21 species, of which eight are used as food, fodder or cover crops (Tomooka et al., 2001, 2002a). In the new system, the subgenus was divided into three sections, Angulares, Ceratotropis and Aconitifoliae consisting of 12, 4 and 5 species, respectively.
The materials used for DNA isolation are agroinoculaed leaves, seeds and embryos.
The supernatant in each step should be carefully transferred without disturbing the other layers.
Repeat the purification steps if there is a presence of phenolics in DNA.
PCR compatible DNA (highly purified DNA) can be isolated by this method.
Sudha Manickam, Unaffiliated
Source: Protocol Exchange (2009) doi:10.1038/nprot.2009.167. Originally published online 8 September 2009.