scientificprotocols authored about 8 years ago
Before you start, have ready the following:
At 2.5 days p.c. (post coitus) the morulae are present in the oviducts. For this reason, it is only necessary to remove the oviducts from the mouse.
Using the watchmaker forceps, manipulate the oviduct so that you have the end of the oviduct (close as possible to the uterotubal junction) between the forceps and insert the flusher so that it points away from the uterotubal junction. Be careful not to pierce through both sides of the oviduct tube. Keeping the flusher inserted into the oviduct, pick up the syringe in the other hand and give a couple of short, sharp squeezes on the plunger. This should flush all morulae from the oviduct. Sometimes, if no morulae are flushed through, it is hard to tell whether the oviduct has been correctly flushed. If there is much debris floating around, then the oviduct has been properly flushed. The morulae are then collected using a mouth pipette and deposited into a petri dish in microdrops of M16 medium. These drops are covered with fluid 200 (Dow Corning, viscosity 50CS) and placed in a 37°C incubator buffered with 5% CO2.