Proteomics

steven4564 authored 4 months ago

Simple Introduction

First of all, let's start with its definition: Peptide mapping is the main method for the analysis and identification of protein products and preparations. In addition, there are some other core techniques such as peptide mass mapping and peptide mapping ms.

The following are functions of Peptide mapping:

To confirm that a protein primary structure (amino acid sequence) including the N and C terminals.

1)To provide protein loci and the proportion of modified groups, such as glycosylation, acetylation, sulphates a00nd phosphorylation. 2)To provide qualitative and quantitative information on protein degradation products 3)To get information on protein oxidation and deamination directly.

According to these useful features above, peptide mapping has already gained in popularity in biological field. After getting numerous data from peptide mapping, the next step is deeper analysis.

A sample graph of peptide mapping

Peptide Mapping Analysis

Based on graphs of peptide mapping and also peptide mass mapping, scientists can get a lot of useful information.According to the size of molecular weight of proteins, peptides and amino acid composition characteristics, the use of strong specificity of proteolytic enzyme [is commonly endopeptidase] on the special peptide chain site will peptide fragment into smaller fragments, through the separation of a certain form characteristic fingerprint detection methods.

The picture below shows the steps of peptide mapping method:

Step 1:

Protein digestion:Immobilized trypsin provides rapid and simple protein digestion with high reproducibility, high sensitivity and excellent data quality in a format that is compatible with automated operations.

Step 2:

Peptide separation:this technique provides a complete set of chromatographic tools for all development applications requiring peptide separation and analysis

Step 3:

Biological mass spectrometer: It provides a fast and easy to solve multicomponent analysis method, Used for sequence determination, structural analysis, molecular weight determination and component content determination of polypeptides. What's more, it has the characteristics of high sensitivity, strong selectivity and good accuracy.

Step 4:

Peptide analysis software: Integrated software can save time and identify more materials. Simple software workflows can guide biotherapy, characterization pathways, and provide comprehensive coverage, including peptide sequence validation and identification of all variants and modifications.

So peptide mapping analysis is quite effective and important in some specific aspect of research. Let's take an example here:

Study on quality control of impurities and harmful substances in Genetic Recombinant Drug is a crucial project., in particular, when the recombinants are used in the production of genetically engineered drugs undergo mutations. Probably, they would bring some mutations into drugs. Besides strengthen control of original material and process of production, peptide mapping analysis is necessary method to ensure safety and consistency.

As a result, peptide mapping analysis has given rise to a number of new things. For instance, HPCE, A new electrical technology for separation which emerged in 1980s. Because of its relatively high resolution, HPCE has started a wider road for structural analysis and quality control of protein drugs.

At present, in addition to the routine analysis of amino acid sequence of some small peptides, the peptide graph analysis is one of the important conventional indicators to control the consistency of most gene engineering products. All in all, technologies on peptide mapping have already penetrated into every corner of our everyday life.

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