3-AMINOPROPYLTRIETHOXYSILANE TREATED SLIDES

• Protocol
• Discussion

Author: University of Nottingham Medical School Division of Histopathy    

METHOD:

1. Wash glass slides in detergent for 30 minutes.
2. Wash glass slides in running tap water for 30 minutes.
3. Wash glass slides in distilled water 2x5 minutes.
4. Wash glass slides in 95% alcohol 2x5 minutes.
5. Air dry for 10 minutes.
6. Immerse slides in a freshly prepared 2% solution for 3-aminopropyltriethoxysilane in dry acetone for 5 seconds.
7. Wash briefly in distilled water twice.
8. Dry overnight at 42C and store at room temperature.

• Note: 300mls of silane solution is sufficient to do 200 slides. Treated slides can be kept indefinitely.